Journal: iScience

Article Title: Circadian disruption exacerbates MASH by reducing Akkermansia muciniphila via the FXR-CYP7A1-bile acid axis

doi: 10.1016/j.isci.2026.115397

Figure Lengend Snippet: Circadian disruption promotes inflammation, lipid accumulation, and fibrosis in MASH mice (A) The relative mRNA expression of lipid-metabolism-related genes ( Srebp , Fasn , and Acaca ), inflammation-related genes ( Cxcl2 , Ccl2 , and Nos2 ), and fibrosis-related genes ( Col3a1 , Col4a1 , and α-Sma ) in MASH with or without circadian disruption, n = 6. (B) Flow cytometry analysis of the abundance of macrophages (CD11b + F4/80 + ), M1 cells (CD86 + CD206 − ), and M2 cells (CD86 − CD206 + ) in the liver in each group, n = 6. (C) Comparison of M1/M2 polarization in the liver in each group by CD86/CD206 detected by immunohistochemistry. Scale bars: 100 μm (10×). (D) Serum IL-6, IL-1β, and TNF-α in each group, n = 6. (E) The relative expression level of the oxidative stress indicator ROS, the content of GSH, and the relative mRNA expression level of Hif-1α related to them in each group, n = 6. (F) The total number of genes detected by liver transcriptomics, as well as the number of genes that are relatively upregulated and downregulated in the two groups. (G) NMDS plot of the main components of liver transcriptomics in each group, n = 4. (H) Reactome analysis results (barplot) of liver transcriptomics in the two groups. (I) Reactome analysis results (dotplot) of liver transcriptomics in the two groups. Data are expressed as mean ± SD ( n = 4–6), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, compared with the MASH group. p values were calculated using unpaired t test. Srebp , sterol regulatory element-binding protein; Fasn , fatty acid synthase; Acaca , acetyl-CoA carboxylase; Cxcl2 , chemokine (C–X–C motif) ligand 2; Ccl2 , chemokine (C–C motif) ligand 2; Nos2 , nitric oxide synthase 2; Col3a1 , collagen alpha-1 (III) chain; Col4a1 , collagen alpha-1 (IV) chain; α-Sma , α-smooth muscle actin; M1, M1 polarization of macrophages; M2, M2 polarization of macrophages; CD11b, marker of mouse macrophages; F4/80, marker of mouse macrophages; CD86, marker of M1 polarization of mouse macrophages; CD206, marker of M2 polarization of mouse macrophages; IL-6, interleukin-6; IL-1β, interleukin-1 beta; TNF-α, tumor necrosis factor-alpha; ROS, reactive oxygen species; GSH, glutathione; Hif-1α , hypoxia inducible factor-1 alpha; NMDS, non-metric multidimensional scaling.

Article Snippet: Rabbit anti-CD206 antibody , Cell Signaling Technology , Cat# 24595; RRID: AB_2892682.

Techniques: Disruption, Expressing, Flow Cytometry, Comparison, Immunohistochemistry, Transcriptomics, Binding Assay, Marker

Journal: Kidney International Reports

Article Title: Pauci-Immune Endocapillary Proliferative Glomerulonephritis With Glomerular M2 Macrophage Infiltration

doi: 10.1016/j.ekir.2026.103791

Figure Lengend Snippet: Multiplex immunofluorescence staining results (Case 3). (a) Macrophage subtypes and their distribution patterns. (b) CD86+ macrophages are sparsely distributed within the renal interstitium. (c–e) CD163+ and CD206+ macrophages are abundantly present in the interstitium, whereas CD163+ macrophages show less infiltration within the glomeruli. The scale bar in the figure represents 20 μm. DAPI, 4',6-diamidino-2-phenylindole.

Article Snippet: Formalin-fixed paraffin-embedded renal tissue sections (3.5 μm) were subjected to multiplex IF staining using the following primary antibodies: CD68 (ab955, pan-macrophage marker, Abcam), CD163 (ab182422, M2 macrophage marker, Abcam), CD86 (91882S, M1 macrophage marker, Cell Signaling Technology, Danvers, MA), CD206 (24595S, M2 macrophage marker, Cell Signaling Technology), CD56 (99746S, Cell Signaling Technology), CD3 (ab11089, Abcam), and CD8 (ab199016, Abcam).

Techniques: Multiplex Assay, Immunofluorescence, Staining

Journal: Materials Today Bio

Article Title: Ultrasound-activated piezoelectric Silk-PVDF hydrogel reprograms the osteoimmune microenvironment via NRF2 signaling for accelerated bone regeneration

doi: 10.1016/j.mtbio.2026.102779

Figure Lengend Snippet: Piezoelectric hydrogel activates NRF2 to attenuate ROS and macrophages polarization for osteogenesis . (A–B) RT-qPCR results for the mRNA expression of pro-inflammatory differentiation of macrophages. (C–D) RT-qPCR results for the mRNA expression of anti-inflammatory differentiation of macrophages. (E) The relative protein expression levels of INOS, CD206. (F–G) Semi-quantitative analysis of immunoblotting results of INOS, CD206. (H) ROS staining of ADSCs. (I) Mean intensity of ROS staining. (J) The relative protein expression levels of NRF2, NQO1, GPX4. (K–M) Semi-quantitative analysis of immunoblotting results of NRF2, NQO1, GPX4. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: Cells were fixed with 4 % paraformaldehyde (Servicebio, #G1101) for 15 min at 25 °C, permeabilized with 0.3 % Triton X-100 in PBS for 15 min, and blocked with 5 % BSA (Sigma, #A7906) containing 10 % normal goat serum (Servicebio, #G5009) for 1 h. Primary antibodies were diluted in antibody diluent (Servicebio, #G1212) and incubated overnight at 4 °C: • Osteopontin (OPN): Rabbit monoclonal (Proteintech, #22952-1-AP), 1:500 • Osteocalcin (OCN): Rabbit polyclonal (Proteintech, #20277-1-AP), 1:500 • iNOS: Mouse anti-iNOS (Proteintech, #22226-1-AP), 1:500 • CD206: Rabbit anti-CD206 (Proteintech, #18704-1-AP), 1:500 After three PBS washes, species-matched secondary antibodies were applied for 1 h at 25 °C in the dark: • Alexa Fluor 488: Goat anti-rabbit IgG (Servicebio, #GB25303), 1:500 • Alexa Fluor 488: Goat anti-mouse IgG (Servicebio, #GB25301), 1:500 • Cy3: Goat anti-rabbit IgG (Servicebio, # GB21303), 1:500 Nuclei were counterstained with DAPI (Servicebio, #G1407).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Staining